Analysis of the cya locus of Escherichia coli |
| |
Authors: | A H Koop M Hartley S Bourgeois |
| |
Affiliation: | Regulatory Biology Laboratory, The Salk Institute, P.O. Box 85800, San Diego, CA 92138 U.S.A. Tel. 619-453-4100, ext. 365 |
| |
Abstract: | A 9500-bp DNA segment containing the adenylate cyclase gene (cya) of Escherichia coli has been isolated and analyzed. Four large proteins are encoded within this fragment - the adenylate cyclase protein (92 kDal), two proteins of unknown function (37 and 32 kDal), and a part of the uvrD-coded protein. Various truncated adenylate cyclase proteins, made from cya genes having as much as 60% of their carboxy-terminal end deleted, are sufficient to complement cya- hosts. When these truncated cya genes are present on a multicopy plasmid in a cya- host, the synthesis of beta-galactosidase is still regulated by glucose. The "maxicell" technique was used to visualize the four proteins encoded by this region and some of the truncated adenylate cyclase proteins. |
| |
Keywords: | Recombinant DNA maxicells modified pKO-1 vector bp base pair CRP cAMP receptor protein adenylate cyclase gene IPTG k kilobase pair ONPG [ ] indicates plasmid-carrier state |
本文献已被 ScienceDirect 等数据库收录! |
|