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Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan
Authors:Ohara Masaru  Kouda Shuntaro  Onodera Makoto  Fujiue Yoshihiro  Sasaki Megumi  Kohara Tadahiro  Kashiyama Seiya  Hayashida Shizue  Kadono Manami  Komatsuzawa Hitoshi  Gotoh Naomasa  Usui Tuguru  Itaha Hideyuki  Kuwabara Masao  Yokoyama Takashi  Sugai Motoyuki
Institution:Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Abstract:Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-beta-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing P. aeruginosa strains in Japanese hospitals.
Keywords:pseudomonas aeruginosa  drug resistance  metallo‐P‐lactamase  molecular epidemiology
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