Physiological and Biochemical Characterization of a Novel Nicotine-Degrading Bacterium Pseudomonas geniculata N1 |
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| Authors: | Yanghui Liu Lijuan Wang Kaiming Huang Weiwei Wang Xueling Nie Yi Jiang Pengpeng Li Shanshan Liu Ping Xu Hongzhi Tang |
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| Affiliation: | 1. State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, People''s Republic of China.; 2. School of Environmental Science and Engineering, Shanghai Jiao Tong University Shanghai, People''s Republic of China.; 3. Shanghai Nuclear Engineering Research & Design Institute, Shanghai, People''s Republic of China.; Massachusetts Institute of Technology, United States of America, |
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| Abstract: | Management of solid wastes with high nicotine content, such as those accumulated during tobacco manufacturing, poses a major challenge, which can be addressed by using bacteria such as Pseudomonas and Arthrobacter. In this study, a new species of Pseudomonas geniculata, namely strain N1, which is capable of efficiently degrading nicotine, was isolated and identified. The optimal growth conditions for strain N1 are a temperature of 30°C, and a pH 6.5, at a rotation rate of 120 rpm min−1 with 1 g l−1 nicotine as the sole source of carbon and nitrogen. Myosmine, cotinine, 6-hydroxynicotine, 6-hydroxy-N-methylmyosmine, and 6-hydroxy-pseudooxynicotine were detected as the five intermediates through gas chromatography-mass and liquid chromatography-mass analyses. The identified metabolites were different from those generated by Pseudomonas putida strains. The analysis also highlighted the bacterial metabolic diversity in relation to nicotine degradation by different Pseudomonas strains. |
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