Infection with Leishmania major Induces a Cellular Stress Response in Macrophages |
| |
| Authors: | Alessandra A Filardy Ana Caroline Costa-da-Silva Carolina M Koeller Kamila Guimar?es-Pinto Flávia L Ribeiro-Gomes Marcela F Lopes Norton Heise Célio G Freire-de-Lima Marise P Nunes George A DosReis |
| |
| Institution: | 1. Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; 2. Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.; 3. Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Federal University of São Paulo, Brazil, |
| |
| Abstract: | We investigated early cellular responses induced by infection with Leishmania major in macrophages from resistant C57/BL6 mice. Infection increased production of reactive oxygen species by resident, but not inflammatory peritoneal macrophages. In addition, infection increased activation of stress-activated protein kinases/c-Jun N-terminal kinases (SAPK/JNK) in resident, but not in inflammatory peritoneal macrophages. Infection also increased expression of membrane and soluble FasL, but infected macrophages remained viable after 48 h. Infection increased secretion of cytokines/chemokines TNF-α, IL-6, TIMP-1, IL-1RA, G-CSF, TREM, KC, MIP-1α, MIP-1β, MCP-1, and MIP-2 in resident macrophages. Addition of antioxidants deferoxamine and N-acetylcysteine reduced ROS generation and JNK activation. Addition of antioxidants or JNK inhibitor SP600125 reduced secretion of KC. Furthermore, treatment with antioxidants or JNK inhibitor also reduced intracellular parasite replication. These results indicated that infection triggers a rapid cellular stress response in resident macrophages which induces proinflammatory signals, but is also involved in parasite survival and replication in host macrophages. |
| |
| Keywords: | |
|
|
