Effects of 2,3,5-Triiodobenzoic Acid on Plantlet Formation from Cultured Tissues of Taro, Colocasia esculenta (L) Schott (Araceae) |
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Authors: | NYMAN, L P ARDITTI, J |
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Affiliation: | Department of Developmental and Cell Biology, University of California Irvine, CA 92717, USA |
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Abstract: | Cultures of a Californian cultivar of Colocasia esculenta varantiquorum, UCI Runner, produced abnormal structures in additionto plantlets on Linsmaier-Skoog (LS) medium supplemented with1 0 mg 11 adenine-N-benzyl-tetrahydro-2H-pyran-2-yl or6-dimethylaminopurine and 0 1 mg 11 napthaleneaceticacid after at least 32 weeks of culture A number of substitutionsand combinations of growth regulators were tested in an attemptto stimulate normal plantlet development These included trialswith 2,4,5-trichlorophenoxyacetic acid, aminocyclopropane-1-carboxylicacid, and 2,3,5-triodobenzoic acid (TIBA) When tissues werecultured on LS medium without hormones, and supplemented with1 mg 11 TIBA, plantlet production occurred in 2 to 4weeks and the number of abnormal structures was reduced Auxin, calloid, callus culture, cytokinins, micropropagation, development |
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