小鼠卵巢组织的超速冻存法研究

目的　本实验通过对小鼠卵巢组织进行冻存研究 ,掌握卵巢的低温生物学特性 ,摸索出一种简便有效的组织器官冻存法 ,为卵巢移植及器官冷冻提供有用的技术方法。方法　通过对小鼠卵巢组织进行慢速程序法与快速液氮蒸汽法冻存 ,比较分析了不同方法所需保护剂种类、浓度、渗透平衡时间。采用对解冻后卵巢组织超微结构观察、组织化学染色、激素测定及自体、异体移植后动情期的恢复作为评价指标。结果与结论　通过上述实验表明用同种冷冻保护剂 ,液氮蒸汽法冻存的卵巢组织超微结构保存良好 ;组织化学染色示其活性与程序法冻存组织相同 ;自体、异体移植后 ,小鼠动情周期的恢复率及血清雌二醇水平各项指标均与慢速程序法冷冻无显著性差异

The Research of Overspeed Method to Cryopreserve Mice Ovarian Tissue

Objective To study the overspeed method of cryopreservation of mice ovarian tissue, and find out a convenient and practical method of its storage. Methods After thawing, the ovarian tissues were transplanted. The recovered time and rate of estrus, level of serum estradiol of recipient mice, enzyme histochemistry of transplantation tissues, the ultrastructure of thawed tissues were studied to compare the cryopretective effect of cryopreservation methods and types of CPA. Results and conclusion In comparison with procedure freezing method,though the same CPA,the liquid nitrogen vapour freezing method showed the advantage of well ultrastructure of ovarian tissue in addition to the same results of enzyme histochemistry.There were no significant difference between the procedure freezing method and liquid nitrogen vapour freezing method in recovered rate of estrus, level of serum estradiol.