Intracellular Localization of Phosphoenolpyruvate Carboxykinase in Bundle Sheath Cells of C4 Plants |
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Authors: | Watanabe Masanao; Ohnishi Jun-ichi; Kanai Ryuzi |
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Institution: | Department of Biochemistry, Faculty of Science, Saitama University Urawa 338, Japan |
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Abstract: | Bundle sheath protoplasts (BSP) were isolated and purified fromfour C4 species of the phosphoenolpyruvate (PEP) carboxykinasetype (Panicum maximum, P. texanum, Chloris gayana and Eriochloaborumensis), and cell organellses were separated from the BSPextract by differential centrifugation or sucrose density gradientcentrifugation. Separation of the organelles was ascertainedby the distribution of marker enzymes for chloroplasts, mitochondria,peroxisomes and cytoplasm. Contrary to the previous report Rathnamand Edwards (1975) Arch. Biochem. Biophys. 171: 214], the distributionof PEP carboxykinase in BSP of P. maximum was the same as thatof UDP-glucose pyrophosphorylase, a marker for cytoplasm, andPEP carboxykinase activity was not recovered in the intact chloroplasts.The same results were obtained with P. texanum, C. gayana andE. borumensis. Therefore, we conclude that PEP carboxykinase is exclusivelylocalized in the cytoplasm of bundle sheath cells of C4 plants. (Received July 23, 1983; Accepted October 17, 1983) |
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