A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P |
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Authors: | Khrouchtchova Anastassia Hansson Maria Paakkarinen Virpi Vainonen Julia P Zhang Suping Jensen Poul Erik Scheller Henrik Vibe Vener Alexander V Aro Eva-Mari Haldrup Anna |
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Affiliation: | Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark. |
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Abstract: | We show that the thylakoid membrane phosphoprotein TMP14 is a novel subunit of plant photosystem I (PSI). Blue native/SDS-PAGE and sucrose gradient fractionation demonstrated the association of the protein exclusively with PSI. We designate the protein PSI-P. The presence of PSI-P subunit in Arabidopsis mutants lacking other PSI subunits was analyzed and suggested a location in the proximity of PSI-L, -H and -O subunits. The PSI-P protein was not differentially phosphorylated in state 1 and state 2. |
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Keywords: | PS, photosystem LHC, light-harvesting complex WT, wild-type Chl, chlorophyll β-DM, dodecyl-β-maltoside BN, blue native |
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