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A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P
Authors:Khrouchtchova Anastassia  Hansson Maria  Paakkarinen Virpi  Vainonen Julia P  Zhang Suping  Jensen Poul Erik  Scheller Henrik Vibe  Vener Alexander V  Aro Eva-Mari  Haldrup Anna
Institution:Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark.
Abstract:We show that the thylakoid membrane phosphoprotein TMP14 is a novel subunit of plant photosystem I (PSI). Blue native/SDS-PAGE and sucrose gradient fractionation demonstrated the association of the protein exclusively with PSI. We designate the protein PSI-P. The presence of PSI-P subunit in Arabidopsis mutants lacking other PSI subunits was analyzed and suggested a location in the proximity of PSI-L, -H and -O subunits. The PSI-P protein was not differentially phosphorylated in state 1 and state 2.
Keywords:PS  photosystem  LHC  light-harvesting complex  WT  wild-type  Chl  chlorophyll  β-DM  dodecyl-β-maltoside  BN  blue native
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