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Bacteriophage mv4 Site-Specific Recombination: the Central Role of the P2 mv4Int-Binding Site |
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| Authors: | Michèle Coddeville Jean-Fran?ois Spinella Pauline Cassart Guillaume Girault Marie-Line Daveran-Mingot Pascal Le Bourgeois Paul Ritzenthaler |
| Affiliation: | aUniversité de Toulouse, Université Paul Sabatier, Laboratoire de Microbiologie et de Génétique Moléculaires, Toulouse, France;bCentre National de la Recherche Scientifique-Unité Mixte de Recherche 5100, Toulouse, France |
| Abstract: | The contributions of the five mv4Int- and two mv4Xis arm-binding sites to the spatial intasome organization of bacteriophage mv4 were found not to be equivalent. The 8-bp overlap region was mapped to the left extremity of the core region and is directly flanked by the P2 Int arm-binding site. These results and the absence of characteristic Int core-binding sites suggest that the P2 site is the determinant for integrase positioning and recognition of the core region. |
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