Wheat V-H+-ATPase Subunit Genes Significantly Affect Salt Tolerance in Arabidopsis thaliana
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Authors: | Xiaoliang He Xi Huang Yinzhu Shen Zhanjing Huang |
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Affiliation: | 1. College of Life Science of Hebei Normal University, Shijiazhuang, Hebei, China.; 2. School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhang, Hebei, China.; University of Nottingham, United Kingdom, |
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Abstract: | Genes for V-H+-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H+-ATPase and Na+/H+ exchange, with the largest increase in plants carrying the c subunit of V-H+-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H+-ATPase subunit in the Arabidopsis increased under salt stress. Overall, our results suggest that each V-H+-ATPase subunit plays a key role in enhancing salt tolerance in plants. |
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