Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis |
| |
| Authors: | Torres-Bacete Jesús Hormigo Daniel Stuart Maribel Arroyo Miguel Torres Pedro Castillón María P Acebal Carmen García José L de la Mata Isabel |
| |
| Affiliation: | Departamento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, C/ José Antonio Nováis 2, 28040 Madrid, Spain. |
| |
| Abstract: | Aculeacin A acylase from Actinoplanes utahensis produced by Streptomyces lividans revealed acylase activities that are able to hydrolyze penicillin V and several natural aliphatic penicillins. Penicillin K was the best substrate, showing a catalytic efficiency of 34.79 mM(-1) s(-1). Furthermore, aculeacin A acylase was highly thermostable, with a midpoint transition temperature of 81.5 degrees C. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
