A GC cluster repeat is a hotspot for mit- macro-deletions in yeast mitochondrial DNA. |
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| Authors: | Georg F Weiller Helmut Bruckner Sang Ho Kim Elke Pratje and Rudolf J Schweyen |
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| Institution: | (1) Institut für Genetik und Mikrobiologie, Universität München, Maria-Ward-Strasse 1a, W-8000 München, FRG;(2) Institut für Mikrobiologie und Genetik der Universität Wien, Althanstrasse 14, A-1090 Vienna, Austria;(3) Institut für Allgemeine Botanik, Universität Hamburg, Ohnehorststrasse 18, W-2052 Hamburg, FRG;(4) Present address: The Australian National University, GPO Box 475, 2601 Canberra City, ACT, Australia;(5) Present address: Europa-Fachpresse, Thomas-Dehler-Strasse 27, W-8000 München 83, Germany;(6) Present address: Department of Biology, Taegu University, 713-714 Kyungbuk Kyungsan-Kun, Seoul, Korea |
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| Abstract: | Summary In a random collection of mit– mutations of the yeast strain 777-3A we find that deletions are exceptionally frequent in the OXI3 gene, a large mosaic gene coding for subunit I of cytochrome oxidase. About 10% of all oxi3
– mutants carry the same macro-deletion, del-A, extending from the 5 non-translated leader of OXI3 to intron 5b of this gene. Determination of the respective wild-type sequences and of the del-A junction sequence revealed that the end-points of the deletion are in two GC clusters with 31 by sequence identity which are located at a distance of 11.3 kb. We speculate that not only the sequence identity of the two GC clusters but also the palindromic structure of these putatively mobile elements of yeast mitochondrial DNA (mtDNA) plays a role in deletion formation. |
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| Keywords: | Yeast mtDNA sequences OXI3 gene mit deletions GC clusters |
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