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Isolation and amplification of DNA from rhizomes of turmeric and ginger
Authors:S Syamkumar  Bosco Lowarence  B Sasikumar
Institution:(1) Genetic Resources and Molecular Breeding Laboratory, Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, 673012 Calicut, Kerala, India
Abstract:Turmeric and ginger are used as spices and in alternative systems of medicine. They are rich in polysaccharides, polyphenols, and alkaloids. A simple and rapid method for isolating good quality DNA with fairly good yields from mature rhizome tissues of turmeric and ginger has been perfected. Isolated DNA was amenable to restriction digestion and PCR amplification.
Keywords:Curcuma            DNA isolation  ginger  RAPD  rhizomes  turmeric            Zingiber
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