The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein. |
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Authors: | T Rajapandi K M Dolan D B Oliver |
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Affiliation: | Department of Microbiology, State University of New York, Stony Brook 11794-8626. |
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Abstract: | TnphoA insertions in the first gene of the Escherichia coli secA operon, gene X, were isolated and analyzed. Studies of the Gene X-PhoA fusion proteins showed that gene X encodes a secretory protein, since the fusion proteins possessed normal alkaline phosphatase activity and a substantial portion of this activity was found in the periplasm. In addition, the Gene X-PhoA fusion proteins were initially synthesized with a cleavable signal peptide. A gene X::TnphoA insertion was used to construct a strain containing a disrupted chromosomal copy of gene X. Analysis of this strain indicated that gene X is nonessential for cell growth and viability and does not appear to play an essential role in the process of protein export. |
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