Inheritance and segregation of exogenous genes in transgenic cotton |
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Authors: | Bao-Hong Zhang Teng-Long Guo Qing-Lian Wang |
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Institution: | (1) Key Laboratory of Cotton Genetic Improvement of the Ministry of Agriculture, Cotton Research Institute, Chinese Academy of Agricultural Sciences, 455112 Anyang Henan, People’s Republic of China;(2) Institute of Heze Agricultural Sciences, Heze, 273000 Shandong, People’s Republic of China;(3) Henan Vocational Technical Teacher’s College, 453003 Xinxiang Henan, People’s Republic of China;(4) 1632 West 6th Street Apt F, 78703 Austin, TX, USA |
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Abstract: | Three transgenic cotton varieties (lines) were chosen for the study of inheritance and segregation of foreign Bt (Bacillus thuringiensis toxin) andtfdA genes in cotton. The transformed cotton varieties CCRI 30 and NewCott 33B expressing the BtcryIA gene, and cotton line TFD expressing thetfdA gene were crossed with CCRI 19, CCRI 12 and Lumian 6. The results confirm inheritance and segregation of (i) the exogenous
Bt gene in transgenic CCRI 30 and NewCott 33B, governing resistance to bollworm, and (ii) the exogenoustfdA gene in transgenic TFD, governing resistance to the herbicide 2,4-D. Both resistance characters were governed by a single
dominant nuclear gene, and were not affected by cytoplasm. Our data support the conclusion that foreign traits encoded by
single genes are inherited and expressed in Mendelian fashion in cotton. Our results also indicate that a practical backcross
breeding program could be used to develop cotton cultivars combining one or more resistance traits from foreign and native
gene sources. |
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Keywords: | transgenic cotton Bt gene tfdA gene inheritance dominant gene |
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