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Low-Dose Automated Electron Tomography: A Recent Implementation |
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| Authors: | BK Rath M Marko M Radermacher J Frank |
| Institution: | aWadsworth Center, New York State Department of Health, Albany, New York, 12201-0509;bWadsworth Center, New York State Department of Health, Albany, New York, 12201-0509, Department of Biomedical Sciences, State University of New York at Albany, 12222, and, Max-Planck-Institut für Biophysik, Kennedyallee 70, D-60596, Frankfurt/M, Germany;cWadsworth Center, New York State Department of Health, Albany, New York, 12201-0509, Department of Biomedical Sciences, State University of New York at Albany, Albany, New York, 12222 |
| Abstract: | Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made. |
| Keywords: | automated electron tomography transmission electron microscopy low-dose microscopy three-dimensional reconstruction triad junction IVEM |
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