光周期影响雌性虹鳟鱼的免疫活动

运用放射免疫测定法，酶联免疫吸附法及酶联免疫斑点法调查了性未成熟雌性虹鳟鱼在生殖季节血浆中的皮质醇，雌二醇，睾丸酮，免疫球蛋白Ｍ（ＩｇＭ），抗体产生细胞等水平的变化，同时也测定了不同光周期条件下ＩｇＭ水平的变化。实验结果表明，伴随生殖季节，血浆中甾醇类激素，ＩｇＭ量和抗体科生细胞数有季节性的变化；另外，生活在不同光周期条件下的虹鳟鱼，血浆中ＩｇＭ的量明显地不同，由于实验是在常温条件下进行的，而且即

IMMUNE FUNCTION OF IMMATURE RAINBOW TROUT (Oncorhynchus mykiss ) RELATED WITH PHOTOPERIOD

Radioimmunoassay,enzyme linked immunospot assay and enzyme immunoassay were used for the determinations of plasma steroid hormone's level,antibody producing cell's counting and IgM level in this study.The decreased number of antibody producing cells and low IgM levels were observed in sexual immature rainbow trout during the spawning season.These fish were reared under almost constant water temperature and natural photoperiod.Moreover,low IgM level was also observed in immature rainbow trout,which were reared under short photoperiod,and IgM level was not changed by treatment of testosterone.The results suggest that photoperoid may cause the changes in immune competence.It is possible that circadian rhythm accompanied with photoperiod may influence physiological function of fish,so that immune competence is changed.