Rabbit enterotoxaemia: Purification and preliminary characterisation of a toxin produced by Clostridium spiroforme |
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| Authors: | D.S. Fernie J.M. Knights R.O. Thomson R.J. Carman |
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| Affiliation: | Department of Bacteriology R and D, Wellcome Research Laboratories, Beckenham, Kent BR3 3BS, U.K.;Animal Unit, Charing Cross Hospital Medical School, St. Dunstans Road, London W6 8RP, U.K. |
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| Abstract: | Abstract A culture filtrate of Clostridium spiroforme grown in a brain-heart-infusion peptone medium at 37°C for 24 h was concentrated by ultrafiltration, and the toxic fraction separated by ion exchange chromatography. This material was dermonecrotic in depilated guinea pigs, lethal in mice, enterotoxic in rabbit ileal loops and infant mice and cytophathic for HeLa cells. All activity was neutralised by Clostridium perfringens Type E antitoxin. Analysis by size exclusion high-performance liquid chromatography produced a single symmetrical peak corresponding to an M r of 41 000 to 42 000. |
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| Keywords: | Lethal necrotic enterotoxin cytotoxin Clostridium |
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