Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form |
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Authors: | Makiko Nakamura Masayasu Mie Hisakage Funabashi Eiry Kobatake |
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Affiliation: | Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. |
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Abstract: | A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could determine nanomolar concentrations of ATP in its fixed form via interactions with biotin-conjugated antibodies. |
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Keywords: | ATP fixed form fusion protein luciferase streptavidin |
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