Basal lamina formation by porcine thyroid cells grown in collagen- and laminin-deficient medium |
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Authors: | Eleonore Fr?hlich Richard Wahl and Klaus Reutter |
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Institution: | (1) Department of Anatomy, Eberhard-Karls University of Tübingen, ?sterbergstr. 3, 72074 Tübingen, Germany;(2) Medical Clinic, Department of Endocrinology, Metabolism and Clinical Chemistry, Eberhard-Karls University of Tübingen, Tübingen, Germany |
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Abstract: | Summary Porcine thyroid cells isolated by dispase treatment were cultured in either (a) Matrigel, (b) agarose with the addition of
different combinations of basic fibroblast growth factor and laminin, or (c) on agarose-coated dishes. The formation of follicles
and the presence of a basal lamina was investigated by routine electron microscopy of Araldite-embedded material and by light
and electron microscopical immunocytochemical detection of the basal lamina components, laminin and collagen type IV. After
10 days of culture in Matrigel or agarose, a basal lamina-like structure surrounded most follicles. Follicles of cells growing
in agarose and overlaid with a medium containing thyrotropin and fibroblast growth factor showed a fluorescent band at the
basal side of the follicles after immunocytochemical staining with anti-laminin and anti-collagen antibodies. Routine electron
microscopy showed that a basal lamina-like structure lined the outside of the follicle. This structure could be subdivided
into a lamina lucida and a lamina densa. Electron microscopical immunogold labelling revealed that immunologically detectable
laminin was confined to the lamina densa. These findings suggest that even in the absence of basal lamina components in the
culture medium, thyroid cells are able to form follicles with a regular basal lamina when they are cultured in a three-dimensional
environment. |
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Keywords: | |
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