Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells |
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| Authors: | Makiko Yamashita Yoshinori Katakura Yoshihiro Aiba Shin-ei Matsumoto Kazuko Morihara Kiichiro Teruya Akira Ichikawa Sanetaka Shirahata |
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| Affiliation: | (1) Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;(2) Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;(3) Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan |
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| Abstract: | Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC. |
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| Keywords: | Antibody production Interleukin-10 CD11c+ dendritic cells In vitro immunization Peripheral blood mononuclear cells |
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