Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment |
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Authors: | Menashe Marcus Ruth Goitein Alfred Gropp |
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Institution: | (1) Department of Genetics, The Hebrew University, Jerusalem, Israel;(2) Institut für Pathologie der Medizinischen Hochschule Lübeck, D-2400 Lübeck, Federal Republic of Germany |
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Abstract: | Summary Condensation of human chromosomes in phase G2 and early mitosis is inhibited by the fluorochrome 33258-Hoechst. This inhibitory effect is most apparent in primary diploid fibroblasts and lymphoblasts and least pronounced in peripheral blood lymphocytes. Condensation of the human Y chromosome, which contains a large heterochromatic region rich in A-T base pairs, is drastically inhibited by 33258-Hoechst treatment of fibroblasts and lymphoblasts. The difference in sensitivity of human chromosomes in different cell types to 33258-Hoechst probably reflects differences in the cell-membrane permeabilities to 33258-Hoechst. |
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