High-frequency somatic embryogenesis from small suspension-cultured clusters of cells of an interspecific hybrid of Oryza |
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Authors: | Kenjirou Ozawa Ding-Hou Ling Atsushi Komamine |
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Institution: | (1) National Institute of Agrobiological Resources, Kannondai, 305 Tsukuba, Japan;(2) South China Institute of Botany, Academia Sinica, 510650 Guanzhou, China;(3) Department of Chemical and Biological Sciences, Japan Women's University, Mejirodai, 112 Tokyo, Japan |
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Abstract: | Suspension cultures in which cell clusters were small and had a high capacity for somatic embryogenesis (about 60%) were established from immature panicles of F1 plants from a cross between Oryza sativa and Oryza latifolia The cells were subcultured at seven-day intervals in a modified N6 medium. The cell clusters were quite small (approximately 30–200 m in diameter) after culture for two months in this medium. When small clusters of cells were transferred to N6 medium, that had been diluted with an equal volume of water and supplemented with -naphthalenacetic acid (53 nM), 4-pyridylurea (2.2 M) and sucrose (30 gl-1), somatic embryogenesis occurred at high frequency (about 60%). The system established in the present work is useful for biochemical and molecular biological research of the somatic embryogenesis in the Gramineae.Abbreviations 2,4-d
2,4-dichlorophenoxyacetic acid
- NAA
-naphthalenacetic acid
- 4-PU
4-pyridylurea
- MS
Murashige and Skoog (1962) |
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Keywords: | Oryza sativa Oryza latifolia |
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