Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation |
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Authors: | Jee In Kim Garbo D Young Li Jin Avril V Somlyo Masumi Eto |
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Institution: | (1) Department of Molecular Physiology and Biophysics, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA;(2) Department of Molecular Physiology and Biological Physics, The University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA;(3) Kimmel Cancer Center, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA |
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Abstract: | Ca2+ sensitivity of smooth muscle (SM) contraction is determined by CPI-17, an inhibitor protein for myosin light chain phosphatase
(MLCP). CPI-17 is highly expressed in mature SM cells, but the expression level varies under pathological conditions. Here,
we determined the expression of CPI-17 in embryonic SM tissues and arterial neointimal lesions using immunohistochemistry.
As seen in adult animals, the predominant expression of CPI-17 was detected at SM tissues on mouse embryonic sections, whereas
MLCP was ubiquitously expressed. Compared with SM α-actin, CPI-17 expression doubled in arterial SM from embryonic day E10
to E14. Like SM α-actin and other SM marker proteins, CPI-17 was expressed in embryonic heart, and the expression was down-regulated
at E17. In adult rat, CPI-17 expression level was reduced to 30% in the neointima of injured rat aorta, compared with the
SM layers, whereas the expression of MLCP was unchanged in both regions. Unlike other SM proteins, CPI-17 was detected at
non-SM organs in the mouse embryo, such as embryonic neurons and epithelium. Thus, CPI-17 expression is reversibly controlled
in response to the phenotype transition of SM cells that restricts the signal to differentiated SM cells and particular cell
types.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Smooth muscle contraction Smooth muscle development Vascular biology Vascular injury CPI-17 Myosin light chain phosphatase Myocardin PKC ROCK |
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