Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction |
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Authors: | Lisa Timmons Hayley LunaJordan Martinez Zachary MooreVaishnavi Nagarajan Juliana Metichecchia KemegeNadeem Asad |
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Institution: | Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA |
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Abstract: | Rare Caenorhabditis elegans males arise when sex chromosome non-disjunction occurs during meiosis in self-fertilizing hermaphrodites. Non-disjunction is a relatively rare event, and males are typically observed at a frequency of less than one in five hundred wild-type animals. Males are required for genetic crosses and phenotypic analysis, yet current methods to generate large numbers of males can be cumbersome. Here, we identify RNAi reagents (dsRNA-expressing bacteria) with improved effectiveness for eliciting males. Specifically, we used RNAi to systematically reduce the expression of over two hundred genes with meiotic chromosome segregation functions, and we identified a set of RNAi reagents that robustly and reproducibly elicited male progeny. |
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Keywords: | RNAi Caenorhabditis elegans males Non-disjunction Kinesin-like protein Him phenotype dsRNA |
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