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Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

Authors:	Ranran Cheng Jin Peng Yonghong Yan Peili Cao Jiewei Wang Chen Qiu Lichun Tang Di Liu Li Tang Jianping Jin Xingxu Huang Fuchu He Pumin Zhang

Institution:	1. State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China;2. The College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100142, China;3. The Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China;4. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hanzhou, Zhejiang Province 310011, China;5. Department of Biochemistry and Molecular Biology, University of Texas Health Sciences Center, Houston, TX 77030, United States;6. Model Animal Research Center, Nanjing University, Nanjing, Jiangsu Province 210061, China;^g National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China;^h Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, United States

Abstract:	We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.

Keywords:	CRISPR/Cas9 Gene editing Liver Adenoviruses
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