Calcium signaling mediates the response to cadmium toxicity in Saccharomyces cerevisiae cells |
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Authors: | Lavinia L. Ruta Valentina C. Popa Ioana Nicolau Andrei F. Danet Virgil Iordache Aurora D. Neagoe Ileana C. Farcasanu |
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Affiliation: | 1. University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania;2. University of Bucharest, Faculty of Biology, Spl. Independentei 91-95, 050095 Bucharest, Romania |
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Abstract: | The involvement of Ca2+ in the response to high Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, and Hg2+ was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca2+ when exposed to Cd2+, and to a lesser extent to Cu2+, but not to Mn2+, Co2+, Ni2+, Zn2+, or Hg2+. The response to high Cd2+ depended mainly on external Ca2+ (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca2+ (released into the cytosol through the Yvc1p channel). The adaptation to high Cd2+ was influenced by perturbations in Ca2+ homeostasis. Thus, the tolerance to Cd2+ often correlated with sharp Cd2+-induced cytosolic Ca2+ pulses, while the Cd2+ sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca2+. |
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Keywords: | cytAEQ, cytosolic aequorin [Ca2+]cyt, cytosolic Ca2+ ER, endoplasmic reticulum Me2+, divalent cation of any of the metals tested MES, 2-(N-morpholino)ethanesulfonic acid RLU, relative luminescence units SD, synthetic dextrose medium SE, standard error YPD, yeast extract-peptone-dextrose medium WT, parental &ldquo wild type&rdquo cells |
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