Ribonomic approaches to study the RNA-binding proteome |
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Authors: | Camilla Faoro Sandro F. Ataide |
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Affiliation: | School of Molecular Biosciences, University of Sydney, NSW, Australia |
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Abstract: | Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known or predicted RNA-binding domains. However, various methods have been recently developed to capture an RNA of interest and comprehensively identify its associated RBPs. In this review, we discuss the RNA-affinity purification methods followed by mass spectrometry analysis (AP-MS); RBP screening within protein libraries and computational methods that can be used to study the RNA-binding proteome (RBPome). |
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Keywords: | RNA-binding proteins (RBPs) Ribonucleoprotein particles (RNPs) Ribonome RNA&ndash protein interactions RNA-centric methods Non-coding RNAs (ncRNAs) |
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