Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells |
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| Authors: | Ryohei Sugahara Hiroaki Mon Jae Man Lee Takahiro Shiotsuki Takahiro Kusakabe |
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| Affiliation: | 1. Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan;2. Insect Growth Regulation Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan |
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| Abstract: | The silkworm Fanconi anemia (FA) pathway is required for normal cellular resistance to mitomycin C (MMC) in silkworms, but little is known about the requirement for repair of other types of DNA damage. Here we report that silkworm cells deficient for FA proteins FancD2 and FancM exhibit normal sensitivities to hydroxyurea (HU) and camptothecin (CPT), although FancM-dependent FancD2 monoubiquitination is induced upon these treatments. Similar results were observed in cells depleted for Rmi1 and Mhf1, which interact with the FancM protein. We also found that Rad51-knockdown cells exhibited normal sensitivity to HU despite induction of double-strand breaks by HU treatment. |
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| Keywords: | FA, Fanconi anemia HR, homologous recombination MMC, mitomycin C HU, hydroxyurea CPT, camptothecin ICL, interstrand cross-link dsRNA, double-stranded RNA |
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