Removal of both Ycf48 and Psb27 in <em>Synechocystis</em> sp. PCC 6803 disrupts Photosystem II assembly and alters Q<sub>A</sub> oxidation in the mature complex期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

*Removal of both Ycf48 and Psb27 in Synechocystis* sp. PCC 6803 disrupts Photosystem II assembly and alters Q_A oxidation in the mature complex**

Authors:	Simon A Jackson John RD HerveyAsher J Dale Julian J Eaton-Rye

Institution:	Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand

Abstract:	The Photosystem II (PS II) assembly factors Psb27 and Ycf48 are transiently associated with PS II during its biogenesis and repair pathways. We investigated the function of these proteins by constructing knockout mutants in Synechocystis sp. PCC 6803. In ΔYcf48 cells, PS II electron transfer and stable oxygen evolution were perturbed. Additionally, Psb27 was required for photoautotrophic growth of cells lacking Ycf48 and assembly beyond the RC47 assembly complex in ΔYcf48:ΔPsb27 cells was impeded. Our results suggest the RC47 complex formed in ΔYcf48 cells is defective and that this deficiency is exacerbated if CP43 binds in the absence of Psb27.

Keywords:	Bis&ndash Tris 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-propane-1 3-diol BSA bovine serum albumin BMF blue measuring flashes BN-PAGE blue-native polyacrylamide gel electrophoresis CP43 43-kDa chlorophyll a-binding protein of the core antenna CP47 47-kDa chlorophyll a-binding protein of the core antenna D1 Photosystem II reaction center protein subunit D2 Photosystem II reaction center protein subunit DCBQ 2 6-dichloro-1 4-benzoquinone DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea ECL enhanced chemiluminescence HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid iD1 intermediate form of the D1 protein OCP orange carotenoid protein OD optical density pD1 precursor form of the D1 protein PDM PratA-defined membrane PS II Photosystem II Q_A primary plastoquinone electron acceptor of PS II Q_B secondary plastoquinone electron acceptor of PS II RC reaction center RCII reaction center pre-complex containing D1 D2 cytochrome b₅₅₉ and Ycf48 RC47 reaction center complex lacking the CP43 protein RMF red measuring flashes SLIC sequence and ligation independent cloning TES N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid Tris 2-amino-2-hydroxymethyl-propane-1 3-diol
本文献已被 ScienceDirect 等数据库收录！

设为首页 | 免责声明 | 关于勤云 | 加入收藏