Single-copy nuclear DNA sequences obtained from noninvasively collected primate feces |
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Authors: | Surridge Alison K Smith Andrew C Buchanan-Smith Hannah M Mundy Nicholas I |
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Affiliation: | Institute of Biological Anthropology, University of Oxford, Oxford, United Kingdom. |
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Abstract: | Noninvasively collected primate feces have been shown to provide a useful source of mitochondrial DNA for sequencing and nuclear microsatellite DNA for size analysis. In this study, single-copy nuclear DNA sequences were obtained from noninvasively collected fecal samples of two species of wild tamarins, Saguinus fuscicollis and S. mystax, in the context of a project on the functional utility of color vision. Noninvasive genotyping of the X-linked opsin gene is important for future studies of selection and adaptation at this locus in a number of primate species. The wide range of techniques that can now be applied successfully to DNA extracted from feces introduces a broad spectrum of potential genetic studies that can be undertaken on primates, without the need for intrusive or invasive methods. |
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Keywords: | fecal DNA extraction tamarin opsin nuclear DNA PCR color vision |
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