Expression of a human proenkephalin a cDNA inEscherichia coli |
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| Authors: | Hans-Jürg Monstein |
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| Institution: | (1) Dept. of Clinical Chemistry, Laboratory of Molecular Biology, KK 6321 Blegdamsvej 9, DK-2100 Copenhagen, Denmark |
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| Abstract: | A 1000 base pair cDNA coding for the entire human proenkephalin A(proA) polypeptide was subcloned into the multifunctional pMPV 2911/ME. coli vector. The recombinant plasmid was found to express an approximately 30 kDa prohormone, which was recognized by a Met-Arg6-Phe2 antibody, directed against the C-terminal part of the enkephalin A prohormone. The expression of human proenkephalin A cDNA should thus permit the rapid purification of unfused recombinant enkephalin A prohormone, which itself may provide a model substrat to identify endoproteolytic processing activities. |
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| Keywords: | Proenkephalin A recombinant protein E coli expression vector DNA |
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