Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors |
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Authors: | Akram Abi Dave Hartig Karl Vorlnder Anqi Wang Stephan Scholl Hans‐Joachim Jrdening |
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Institution: | Akram Abi,Dave Hartig,Karl Vorländer,Anqi Wang,Stephan Scholl,Hans‐Joachim Jördening |
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Abstract: | Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 gLaminaribiose gsugar?1 in the desorbate with a total productivity of 5.6 mgLaminaribiose Lenzyme bed?1 h?1 without the use of recycles. |
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Keywords: | Adsorption Continuous packed bed reactor Downstream processing Laminaribiose Zeolite BEA |
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