三叶木通(Akebia trifoliata)果实乙醇提取物对酪氨酸酶体外活性的影响

以人工栽培条件下三叶木通(Akebia trifoliata)果实为试材,用80%乙醇分别提取果皮、果肉、种子中的有效成分,经离心过滤后,于旋转蒸发仪上减压蒸馏浓缩,再经真空干燥处理得到三叶木通乙醇提取物。以L-DOPA为底物,熊果苷作阳性对照,分析测定了该提取物对酪氨酸酶活性的抑制百分率。结果表明:不同部位的三叶木通果实乙醇提取物对酪氨酸酶活性均有一定的抑制作用,其中果肉对酪氨酸酶活性的抑制作用最佳,中等浓度的果肉乙醇提取物(200μg/mL)与270μg/mL的熊果苷对酪氨酸酶活性的抑制率相当。

The Inhibitory Effects in vitro of Ethanol Extracts from the Fruit of Akebia trifoliata on the Tyrosinase Activity

Fruits of cultivated Akebia trifoliata were used as experimental materials and the active components were extracted with 80% ethanol from pericarp,flesh and seeds respectively.The ethanol extracts were obtained by centrifuging and filtering,distilling and concentrating on revolving evaporation apparatus at reduced pressure,and then vacuum drying.The inhibitory activities of these extracts on tyrosinase were examined with LDOPA as substrate and arbutin as positive control.The results showed that the extracts from different parts of akebia fruit all had obvious inhibitory activity on tyrosinase,the strongest effects occurred with flesh extract.Flesh extract at 200 μg/mL had similar level of effects to arbutin at 270 μg/mL concentration.