Synthesis of colony-stimulating factor (CSF) and differentiation-inducing factor (D-factor) by osteoblastic cells, clone MC3T3-E1 |
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| Authors: | Y Shiina-Ishimi E Abe H Tanaka T Suda |
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| Institution: | 1. Department of Engineering Science, Trinity University, San Antonio, TX 78212, United States;2. Biomedical Engineering Department, Rensselaer Polytechnic Institute, Troy, NY 12180, United States;3. Neuroscience Program, Trinity University, San Antonio, TX 78212, United States;1. School of Archaeology and Anthropology, Australian National University, 44 Linnaeus Way, Canberra, ACT, 2601 Australia;2. School of Culture, History, and Language, Archaeology and Natural History, College of Asia and the Pacific, Australian National University, Canberra, ACT, 0200, Australia;3. ARC Centre of Excellence for Australian Biodiversity and Heritage, Australian National University, Canberra, ACT, 0200, Australia;4. Bone Biology and Disease Unit, St. Vincent’s Institute of Medical Research, 9 Princes Street, Fitzroy, Melbourne, VIC, 3065, Australia;5. Department of Medicine, The University of Melbourne, St. Vincent’s Hospital, Melbourne, VIC, 3065, Australia;6. MRC Protein Phosphorylation and Ubiquitylation Unit, James Black Centre, University of Dundee, Dundee, DD1 4HN, United Kingdom;7. Infrared Microspectroscopy (IRM) Beamline, ANSTO – Australian Synchrotron, 800 Blackburn Road, Clayton, VIC 3168, Australia;8. Archaeology Division, National Museum of the Philippines, P. Burgos St., Manila, 1000, Philippines;9. Department of Archaeology, University of Aberdeen, St. Mary''s, Elphinstone Road, Aberdeen, AB24 3UF, Scotland, United Kingdom |
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| Abstract: | The role of osteoblasts in inducing the proliferation and differentiation of bone marrow cells was examined. Conditioned medium obtained from mouse osteoblastic cell (MC3T3-E1) cultures stimulated colony formation of mouse bone marrow cells (CSF) and differentiation of mouse myeloid leukemia cells (M1) into macrophage-like cells (D-factor). The CSF activity increased time dependently in parallel with the increase of alkaline phosphatase activity during the culturing of the MC3T3-E1 cells. The activity of the D-factor attained a maximum on days 12 - 15 and decreased thereafter. Both the CSF and the D-factor were eluted in a range of 25,000 to 67,000 daltons on gel filtration. The fraction containing both factors exhibited bone-resorbing activity. These results suggest that osteoblasts are involved in bone resorption at least in part by enhancing the proliferation and differentiation of osteoclast progenitors. |
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