New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis |
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Authors: | David Herthnek Göran Bölske |
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Affiliation: | (1) Department of Bacteriology, National Veterinary Institute (SVA), 751 89 Uppsala, SE, Sweden |
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Abstract: | Background Johne's disease, a serious chronic form of enteritis in ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). As the organism is very slow-growing and fastidious, several PCR-based methods for detection have been developed, based mainly on the MAP-specific gene IS900. However, because this gene is similar to genes in other mycobacteria, there is a need for sensitive and reliable methods to confirm the presence of MAP. As described here, two new real-time PCR systems on the IS900 gene and one on the F57 gene were developed and carefully validated on 267 strains and 56 positive clinical faecal samples. |
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