| 彩色大白菜子叶的不定芽再生 |
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| 引用本文： | 屈会玲,张鲁刚,范爱丽. 彩色大白菜子叶的不定芽再生[J]. 广西植物, 2007, 27(5): 748-754 |
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| 作者姓名： | 屈会玲 张鲁刚 范爱丽 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨凌,712100;山西省运城市农业局,山西,运城,044000;西北农林科技大学,园艺学院,陕西,杨凌,712100 |
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| 基金项目： | 国家863项目(2003AA207120)，陕西省科技攻关项目(2005K01-G8-01)~~ |
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| 摘 要： | 以彩色大白菜子叶为外植体,研究不同激素配比和AgNO3对不定芽再生的影响。结果表明:单独附加细胞分裂素(6-BA或TDZ)的MS培养基,不能诱导子叶不定芽分化;而同时附加生长素(NAA)和细胞分裂素(6-BA或TDZ),不定芽的再生频率提高,最高为15%;AgNO3与细胞分裂素及生长素配合使用,能大幅度提高子叶不定芽的再生频率,提高率最高达42.5%。与6-BA相比,TDZ对不定芽再生的效果更好。当TDZ浓度为0.05mg/L、NAA为0.3mg/L、AgNO3为8mg/L时,产生丛状芽数目最多,再生率最高,达50%。
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| 关 键 词： | 彩色大白菜 子叶片 再生 |
| 文章编号： | 1000-3142（2007）05-0748-07 |
| 修稿时间： | 2007-01-08 |
Shoot regeneration from cotyledon of orange-heading Chinese Cabbage |
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| QU Hui-Ling,ZHANG Lu-Gang,FAN Ai-Li. Shoot regeneration from cotyledon of orange-heading Chinese Cabbage[J]. Guihaia, 2007, 27(5): 748-754 |
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| Authors: | QU Hui-Ling ZHANG Lu-Gang FAN Ai-Li |
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| Affiliation: | 1. College of Horticulture,Northwest Agriculture and Forestry University, Yangling 712100, Chinas ;2. Agricultural Bureau of Yuncheng City, Yuncheng 044000, China |
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| Abstract: | The effects of hormone combination and AgNO3 on adventitious shoot regeneration from cotyledon of orange-heading Chinese Cabbage were studied. The results showed that the adventitious shoot could not be induced from the cotyledon in MS medium added with cell division hormone(6-BA or TDZ)only;the regeneration frequency of shoot reached to 15% in MS medium added with cell division hormone(6-BA or TDZ)and growth hormone(NAA)together;the regeneration frequency could increase 42.5% in MS medium added with cell division hormone(TDZ),growth hormone(NAA)and AgNO3. Compared with 6-BA,TDZ had more effective in inducing shoot regeneration. The maximum frequency of shoot regeneration(50%)was obtained in MS medium which contained 0.05 mg/L TDZ,0.3 mg/L NAA and 8 mg/L AgNO3. |
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| Keywords: | Brassica campestris ssp.pekinensis cotyledon regeneration |
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