Online monitoring of oxygen in spinner flasks |
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| Authors: | Rahul Ravi Deshpande Elmar Heinzle |
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| Institution: | (1) Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123 Saarbrücken, Germany;(2) Present address: Department of Biochemical and Chemical Engineering, University of Dortmund, Emil-Figge-Strasse 66, 44227 Dortmund, Germany |
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| Abstract: | We show the application of a novel optical on-line sensor fixed in spinner flasks for the online monitoring of dissolved O2 concentrations during mammalian cell growth. Using this sensor that requires only minute changes to the flask to be made,
we could determine the volumetric O2 transfer coefficient as well as O2 consumption rates. Under normal growth conditions the cells did not undergo O2 limitation. Also, the transfer of O2 from the atmosphere to the spinner flasks is influenced by the use of screw caps. The on-line measurement was further applied
to determine the O2 uptake rates which can then be used to monitor the metabolic state of the cells and also for online process monitoring. |
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| Keywords: | Mammalian cell culture Online O2 monitoring Optical oxygen sensor Oxygen transfer Spinner flask |
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