Restoration of the acute phase response after infection in cyclophosphamide-treated mice by granulocyte colony-stimulating factor |
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Authors: | Masayoshi Sawaki Eiji Kita Keiichi Mikasa Mitsuru Konishi Mikikazu Kumimatsu Shuzo Kashiba Nobuhiro Narita |
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Institution: | (1) Dept. of Internal Medicine II, Nara Medical University, 840, Shijyocho, Kashihara, 634 Nara, Japan;(2) Dept. of Bacteriology, Nara Medical University, 840, Shijyocho, Kashihara, 634 Nara, Japan |
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Abstract: | The therapeutic effect of granulocyte colony-stimulating factor (G-CSF) against intramuscular infection withPseudomonas aeruginosa in cyclophosphamide (CY)-treated mice was analyzed by measuring plasma levels of amyloid P-component (APC) and proinflammatory cytokine levels. CY (100mg/kg) treatment of mice significantly suppressed plasma concentrations of APC and tumor-necrosis factor- (TNF- ) following infection withP. aeruginosa, in associated with enhanced susceptibility of the treated mice to this bacterium. A 4-day treatment of CY-treated mice with recombinant human G-CSF (rhG-CSF) increased resistance of CY-treated mice, together with the marked restoration of APC and TNF- productions. The capacity to produce interleukin 1- and TNF- of peritoneal macrophages and also that to produce IL-6 of spleen cells were significantly enhanced by thein vivo administration of rhG-CSF in CY-treated mice. These results indicate that G-CSF may increase the functions of monocytes/macrophages directly or indirectlyin vivo. Therefore, the therapeutic effect of rhG-CSF seems to consist of not only increases in the number and functions of neutrophills but also enhancement of monocyte/macrophage functions.Abbreviations rhG-CSF
recombinant human granulocyte-colony stimulating factor
- PMNs
polymorphonuclear leukocytes
- CY
cyclophosphamide
- HBSS
Hanks' balanced salt solution
- APC
amyloid P-component
- IEP
immunoelectrophoresis
- CFU
colony-forming units
- TNF-
tumor-necrosis factor-
- d IL
interleukin |
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Keywords: | G-CSF acute phase response P aeruginosa amyloid P-component cyclophosphamid |
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