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Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling |
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| Authors: | Tanaka Katsunori Yokoi Satomi Morimoto Koji Iwata Takayuki Nakamoto Yuka Nakayama Kaori Koyama Koichi Fujiwara Takeshi Fukase Koichi |
| Affiliation: | Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka-shi, Osaka 560-0043, Japan. |
| Abstract: | Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody. |
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