Expression of human growth hormone in transgenic rice cell suspension culture |
| |
Authors: | Tae-Geum Kim Moon-Yeoun Baek Eun-Kyung Lee Tae-Ho Kwon Moon-Sik Yang |
| |
Institution: | (1) Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University, Jeonju, 561-756, South Korea;(2) Unhwa Corp, Jeonju, 561-211, South Korea;(3) Jeonju Biomaterials Institute, Jeonju, 561-360, South Korea |
| |
Abstract: | Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein
synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized
codon usage via an overlap PCR strategy and located in a plant expression vector under the control of the rice amylase 3D
(Ramy3D) promoter, which is induced by sugar starvation. The plant expression vector was introduced into rice calli (Oryza sativa L. cv. Donjin) via particle bombardment transformation methods. The integration of the shGH gene into the chromosome of the
transgenic rice callus was verified via genomic DNA PCR amplification and shGH expression in transgenic rice suspension cells
was confirmed via Northern blot analysis. The shGH protein was detected in the transgenic rice cell suspension culture medium
following induction with sugar starvation, using Western blot analysis. The quantity of shGH that accumulated in the transgenic
rice cell suspension medium was 57 mg/l. The shGH accumulated in the transgenic rice cell suspension culture medium evidenced
a biological activity similar to that of Escherichia coli-derived recombinant hGH. These results indicate that the shGH was generated and accumulated in the transgenic rice cell suspension
culture medium, and manifested biological activity. |
| |
Keywords: | Human growth hormone Rice cell suspension culture Rice amylase 3D promoter |
本文献已被 PubMed SpringerLink 等数据库收录! |
|