Immobilization and nonspecific adsorption of proteins to pyrogeneous highly dispersed silicon dioxide |
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| Authors: | M Fusek M ?apka J Turková |
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| Institution: | (1) Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6, (Czechoslovakia);(2) Institute of Chemical Process Fundamentals, Czechoslovak Academy of Sciences, 165 02 Prague 6, (Czechoslovakia) |
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| Abstract: | Summary A support based on pyrogeneous silicon dioxide of particle size 0.01 to 0.1/um, modified by 3-(amino)propyltriethoxysilane and activated by glutaraldehyde was employed for the immobilization of concanavalin A, immunoglobulins, basic pancreatic trypsin inhibitor, and chymotrypsin. Its binding capacity is comparable with that of porous supports while the biological activity of the proteins immobilized is retained. Nonspecific adsorption of these proteins to the support is low compared to its binding capacity. |
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