Regeneration of transgenic,microspore-derived,fertile barley |
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| Authors: | A Jähne D Becker R Brettschneider H Lörz |
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| Institution: | (1) Universität Hamburg, Institut für Allgemeine Botanik, AMP II, Ohnhorststrasse 18, D-22609 Hamburg, Germany;(2) Present address: Resistenzlabor der Saaten-Union, Hovedisser Strasse 92, 33818 Leopoldshöhe, Germany |
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| Abstract: | We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue. Independent transformation events led, on average, to the recovery of one plant per 1×107 bombarded microspores. Putative transformants have been regenerated using phosphinothricin as a selective agent. R0 plants have been transferred to soil approximately 2 months after bombardment. Integration of the marker genes bar and uidA has been confirmed by Southern analysis. The marker genes are inherited in all progeny plants confirming the expected homozygous nature of the R0 plants. |
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| Keywords: | Barley microspore culture Particle bombardment Fertile transgenic barley plants |
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