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| 谷氨酸对嗜铬细胞瘤细胞热休克蛋白70 mRNA的诱导分析 | |
| 作者姓名： | Wu BY Lou SJ Xu RB Chen YZ |
| 作者单位： | 1. 上海生命科学研究中心,上海,200031 2. 第二军医大学生理学教研室,上海,200433 3. 第二军医大学生理学教研室病理生理学教研室,上海,200433 |
| 摘 要： | 热休克反应普遍存在于从细菌到人的整个生物界。除热外,多种应激原都可引起热休克蛋白的诱导。至于神经递质是否能够诱导热休克蛋白的表达,目前并不清楚。本文以诱导型热休克蛋白（ｈｓｐ）７０的ｃＤＮＡ为探针,运用Ｎｏｒｔｈｅｒｎ ｂｌｏｔ的方法,在嗜铬细胞瘤细胞（ＰＣ１２）上,分析了谷氨酸和乙酰胆碱对ｈｓｐ７０ ｍＲＮＡ的诱导作用。在此基础上又初步分析了起作用的递质受体。结果表明：在一定的浓度（５０￣５００ |
| 关 键 词： | 热休克蛋白 神经递质 嗜铬细胞瘤 谷氨酸 |
| 修稿时间： | 1997年6月10日 |
Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells |
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| Wu BY,Lou SJ,Xu RB,Chen YZ.Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells[J].Acta Physiologica Sinica,1998,50(5):557-562. | |
| Authors: | Wu B Y Lou S J Xu R B Chen Y Z |
| Institution: | Department of Physiology, Second Military Medical University, Shanghai 200433. |
| Abstract: | The heat shock response has been found in many strains of bacteria to human beings. Besides heat stimuli, many kinds of factors could also induce the synthesis of heat shock proteins (hsp). It is still unknown whether neurotransmitter could induce the increase of hsp expression in mammalian cells. In the present study, the effects of glutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cells were studied by Northern blot method. The probe used is specific for inducible hsp70 mRNA. Our results showed that the glutamic acid under limited conditions (such as at 50-500 mumol/L and action time 5-30 min) could induce the expression of hsp70 mRNA, which was partly mediated by NMDA receptors. On the other hand, ACh (0.1-1,000 mumol/L) could not induce the expression of hsp 70 mRNA. |
| Keywords: | heat shock proteins neurotransmitters PC12 cells glutamic acid acetylcholine |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |