Production of a functional full-length Xenopus laevis c-Myc protein in insect cells |
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Authors: | Jean-Marc Lemaî tre, St phane Bocquet, Nicole Thierry, Robin Buckle,Marcel Mechali |
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Affiliation: | Molecular Embryology Unit, Institut Jacques Monod, 75251, Paris Cedex 05, France |
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Abstract: | C-Myc is a nuclear phosphoprotein whose normal cellular function has not yet been clearly defined. Studies with this protein have always been constrained by the difficulty of obtaining full-length c-Myc in an active form, whatever the expression system used. We report here experimental conditions optimized to increase the solubility and the purification of c-Myc in a baculovirus expression system. Such conditions allow the production of both soluble and active fulllength c-Myc. Interestingly, soluble c-Myc is found associated with a 500-kDa high-molecular-mass complex comparable to that found in human and Xenopus laevis embryos, and which may be required for its function in vivo. |
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Keywords: | Baculovirus cloning system inclusion bodies c-Myc solubility protein complexes DNA-binding activity |
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