| Bt群体信号应答因子nprR基因的缺失对cry1Ac基因表达的影响 |
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| 引用本文： | 王壵,邓超,彭琦,陈榛,张杰,黄大昉,宋福平. Bt群体信号应答因子nprR基因的缺失对cry1Ac基因表达的影响[J]. 微生物学报, 2010, 50(11): 1550-1555 |
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| 作者姓名： | 王壵 邓超 彭琦 陈榛 张杰 黄大昉 宋福平 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所农业病虫害生物学国家重点实验室,北京,100193
2. 中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | “973项目”（2009CB118902） |
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| 摘 要： | 摘要：【目的】研究群体信号应答蛋白编码基因nprR在苏云金芽胞杆菌（Bacillus thuringiensis，Bt）HD-73菌株晶体蛋白形成过程中的作用。【方法】通过同源重组，构建了HD-73 nprR基因缺失突变菌株HD73（ΔnprR ）。利用启动子-lacZ融合、SDS-PAGE方法，测定不同培养基中nprR基因转录活性及nprR基因缺失对cry1Ac转录及表达的影响。【结果】启动子转录活性分析表明，在LB和SSM培养基中nprR基因从对数期结束（T0）开始表达，稳定期持续表达。在LB培养基中，nprR基因的缺失使cry1Ac基因在生长过渡期和稳定期前期转录活性显著提高，同时HD73（ΔnprR ）菌株Cry蛋白生成量也明显高于出发菌株HD-73，但是在芽胞形成释放后，Cry蛋白的表达没有明显的区别。【结论】在丰富培养基中苏云金芽胞杆菌nprR基因的缺失在生长过渡期和稳定期前期能够提高cry1Ac基因转录和表达，从而缩短了cry基因表达时间，并且Cry蛋白总产量与出发菌株相当。
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| 关 键 词： | 关键词：苏云金芽胞杆菌；群体效应；nprR基因；cry1Ac基因； |
| 收稿时间： | 2010-04-22 |
| 修稿时间： | 2010-06-18 |
Effect of Quorum Sensing response regulator nprR deletion on expression of Cry protein in Bacillus thuringiensis |
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| Yao Wang,Chao Deng,Qi Peng,Zhen Chen,Dafang Hang,Jie Zhang and Fuping Song. Effect of Quorum Sensing response regulator nprR deletion on expression of Cry protein in Bacillus thuringiensis[J]. Acta microbiologica Sinica, 2010, 50(11): 1550-1555 |
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| Authors: | Yao Wang Chao Deng Qi Peng Zhen Chen Dafang Hang Jie Zhang Fuping Song |
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| Affiliation: | State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;Biotechnology Research Institute, Chinese Academy of Agricultal Sciences, Beijing 100081, China;State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | |
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| Keywords: | Keywords: Bacillus thuringiensis Quorum Sensing nprR gene cry1Ac gene |
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