Cellulose depolymerization to glucose and other water soluble polysaccharides by shear deformation and high pressure treatment |
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Authors: | Arnis Kokorevics Janis Gravitis |
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Institution: | (1) Latvian State Institute of Wood Chemistry, 27 Dzerbenes St., LV-1006 Riga, Latvia |
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Abstract: | The simultaneous action of shear deformation and high pressure (SDHP) creates changes in the structure of wood and its main
components (cellulose, hemicelluloses, lignin). The formation of water and alkali soluble polysaccharides under SDHP action,
proceeds in seconds in the solid state, without the use of any reagents and solvents. Therefore, SDHP seems to be a technologically
safe method and friendly to the environment. The amorphization of cellulose crystallites and depolymerization of cellulose
chains were observed under a wide range of pressures (1–6 GPa), both for cellulose samples and the cellulose part of wood.
Similar depolymerization occurs in the hemicellulose part of wood. The decomposition of polysaccharides under SDHP causes
the formation of the water soluble part, whose content increases with pressure and the applied shear deformation. A maximum
solubility of 40% and 55% was registered at 6 GPa following treatment of cellulose and birch wood samples. A higher output
in the case of wood can be explained by a specific role of lignin under SDHP, which acts as a ‘grinding stone’ during cellulose
and hemicelluloses destruction. As shown by high-performance size exclusion chromatography, the water soluble fraction obtained
from cellulose contained glucose (2.6%), cellobiose (9.6%), cellotriose (16.6%) and other higher water soluble oligomers (71%).
Almost complete dissolution (98%) of the treated cellulose sample can be achieved by extraction with 10% NaOH solution. The
SDHP treated birch wood was subjected to submerged fermentation (with Trichoderma viride), and a 13% output of proteins was
obtained. In this case, the water soluble part played the role of the so called ‘start sugars’. Abbreviations: ASF, alkali
soluble fraction; DP, degree of polymerization; EC, energy consumption; HP, high pressure; LMWS, low molecular weight sugars;
MC, moisture content; MCC, microcrystalline cellulose; SD, shear deformation, SDHP, shear deformation under high pressure;
SS, shear strength; WSF, water soluble fraction
This revised version was published online in November 2006 with corrections to the Cover Date. |
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Keywords: | glucose polysaccharides wood cellulose lignin high pressure shear deformation depolymerization solubility fermentation |
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