Measuring the toxic effects of high gene dosage on yeast cells |
| |
Authors: | J. Daniel |
| |
Affiliation: | (1) Centre National de la Recherche Scientifique, Centre de Génétique Moléculaire, Laboratoire associéà l’Université Pierre et Marie Curie, 91198 Gif-sur-Yvette, France, FR |
| |
Abstract: | A novel method, which is rapid, reliable and quantitative, is presented for measuring the toxic effects on yeast cells of high dosage of any given gene. It is based on the possibility of monitoring the presence in cells of a plasmid carrying the ADE2 gene from Saccharomyces cerevisiae by direct observation of colonies, the construction of this particular plasmid being easily made by marked homologous recombination in yeast. Four yeast regulatory genes tested were found to result in various degrees of toxicity at high dosage. Possible implications of the measurement of gene toxicity for eukaryotic cell regulatory mechanisms and for the use of novel general approaches to gene selection, such as the gene-gene interference method, are discussed. Received: 18 March 1996 / Accepted: 23 August 1996 |
| |
Keywords: | Eukaryotic regulatory genes ADE2 gene Marked homologous recombination Gene-gene interference Saccharomyces cerevisiae |
本文献已被 SpringerLink 等数据库收录! |
|