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Immunoelectron microscopic localization of calmodulin in corn root cells
作者姓名:LIJIAXU  JIEWENLIU  DAYESUN
作者单位:[1]DepartmentofBiology,HebeiNormalUniversity,Shijiazhuang050016,China [2]InstituteofHematology,ChineseAcademyofMedicalSciences,Tianjin300020,China
摘    要:Methods for the localization of plant calmodulin by immuno-gold and immuno-peroxidase electron microscopy have been developed. In both corn root-cap cells and meristematic cells, calmodulin was found to be localized in the nucleus, cytoplasm, mitochondria as well as in the cell wall, In the meristematic cells, calmodulin was distinctly localized on the plasma membrane, cytoplasmic face of rough endoplasmic rcticulum and polyribosomes. Characteristically, calmodulin was present in the amyloplasts of root-cap cells. The widespread distribution of calmodulin may reflect its plciotropic functions in plant cellular activities.

关 键 词:玉米  根细胞  电子显微镜  免疫定位  钙调蛋白  钙调素

Immunoelectron microscopic localization of calmodulin in corn root cells
LIJIAXU JIEWENLIU DAYESUN.Immunoelectron microscopic localization of calmodulin in corn root cells[J].Cell Research,1993,3(1):12-19.
Authors:LI JIAXU  JIEWEN LIU  DAYE SUN
Institution:LI JIAXU,JIEWEN LIU,DAYE SUN Department of Biology,Hebei Normal University,Shijiazhuang 050016,China Institute of Hematology,Chinese Academy of Medical Sciences,Tianjin 300020,China
Abstract:Methods for the localization of plant calmodulin by immuno-gold and immuno-peroxidase electron microscopy have been developed. In both corn root-cap cells and meristematic cells, calmodulin was found to be localized in the nucleus, cytoplasm, mitochondria as well as in the cell wall. In the meristematic cells, calmodulin was distinctly localized on the plasma membrane, cytoplasmic face of rough endoplasmic reticulum and polyribosomes. Characteristically, calmodulin was present in the amyloplasts of root-cap cells. The widespread distribution of calmodulin may reflect its pleiotropic functions in plant cellular activities.
Keywords:calmodulin  corn root cells  immunoelectron-microscopic localization  
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